qPCR vs. MCA Tests

Executive Summary

Aquaculture is rapidly evolving to meet global protein demands while minimizing environmental impact. This whitepaper explores the integration of AB PALU – a targeted multi-enzyme blend produced by aseptic fermentation of Bacillus subtilis.  AB PALU contains stabilized enzymes produced during fermentation including – Protease, Amylase, Urease, and Lipase. Used in aquaculture, AB PALU can improve nutrient digestibility, reduce waste outputs, and enhance growth performance in farmed fish and shrimp. The blend offers a biologically sustainable alternative to traditional feed additives, aligning with circular economy principles and precision nutrition strategies.

Introduction

  •  Context: With fishmeal prices rising and sustainability pressures mounting, aquaculture is shifting toward plant-based and alternative feed ingredients.
  • Challenge: These ingredients often contain anti-nutritional factors and complex macromolecules that hinder digestibility.
  • Solution: Enzyme supplementation can pre-digest feed components, improving nutrient bioavailability and reducing environmental load.

Enzyme Functions and Synergy

Enzyme Substrate Targeted Function in Aquaculture Benefits
Protease Proteins Hydrolyzes peptide bonds Improves amino acid absorption, reduces nitrogen excretion
Amylase Starch Breaks down polysaccharides Enhances energy availability from plant-based feeds
Urease Urea Converts urea to ammonia Supports nitrogen cycling, reduces ammonia spikes
Lipase Lipids Hydrolyzes fats into fatty acids Improves lipid utilization, supports growth and pigmentation

 

These enzymes act synergistically to optimize digestion across macronutrient classes, especially in early life stages when endogenous enzyme production is limited.

Performance Outcomes

  •  Growth Metrics: Studies show improved feed conversion ratios (FCR), weight gain, and protein efficiency ratios (PER) with enzyme supplementation.
  • Environmental Impact: Reduced nitrogen and phosphorus excretion, leading to better water quality and lower eutrophication risk.
  • Economic Feasibility: Lower reliance on fishmeal, improved feed efficiency, and reduced mortality rates contribute to cost savings.
  1. Sustainability and Regulatory Considerations
  • Eco-Friendly: Enzymes are biodegradable and reduce the need for antibiotics and chemical additives.

Conclusion

The strategic use of a multi-enzyme blend in aquaculture feed represents a powerful tool for improving nutrient utilization, reducing waste, and supporting sustainable growth. As enzyme technologies advance, their role in precision aquaculture will only expand—offering a biologically elegant solution to some of the industry’s most pressing challenges.